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͸ײĿԭzy(c)ԇУø“(lin)߷_ϺsʢˎI(y)ɷ޹˾
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͸ײĿԭzy(c)ԇУø“(lin)߷

͸ײĿԭzy(c)ԇУø“(lin)߷

a(chn)ƷQ(chng)

ͨQ(chng):͸ײĿԭzy(c)ԇУø“(lin)߷


bҎ(gu)

96˷/


A(y);

a(chn)Ʒwⶨԙzy(c)ѪѪ{еı͸ײxĿԭ

pwAø“(lin)߷ԭELISAԙzy(c)ѪѪ{Ʒеı͸ײxĿԭozy(c)

͸ײHepatitis C VirusHCVݔѪ󲡶ԷǼ׷͸׵ҪԭҪͨ^(gu)ݔѪo}Ҳͨ^(gu)н|ĸĿǰȫs1.31.7|˞ԱβȾÿ35f(wn)c͸P(gun)ĸK(j)Ӌ(j)ÿİf(wn)˸Ⱦβ

βȾЕr(sh)(hu)(do)¼԰Yć(yn)س̶ȿȾβпֻF(xin)p΢YҲܽKЇ(yn)Ըβ܌(do)¸Ӳ͸ΰȾs80%˲(hu)F(xin)κΰYܳF(xin)ļ԰Yl(f)ᡢȫʳ½ġIʹɫɫ׃\P(gun)(ji)ʹS㣨Ƥw۰װl(f)SԸȾ߳F(xin)Yܱ仼ڸβһ15~20%ҵHCVȾߞԸȾ^(gu)80~85%ĸȾ߰l(f)չԱ͸ף20%Ŀɰl(f)չwSK4~5%ĸwS߰l(f)μ(x)ԸΰΣʮև(yn)͸ɱβĸβ

͸׺Ŀԭzy(c)½l(f)չһNHCVȾęzy(c)ڸȾHCVHCVĿԭF(xin)^cѪHCV RNAdP(gun)HCVȾ򿵏(f)ʧˣHCVȾ\I(xin)ѪTYHCVȾ\ࡢΣȺO(jin)y(c)߹ϵKߵHCVȾ\beȾֻF(xin)YȾڿЧA(y)y(c)ͱO(jin)y(c)


z(yn)ԭ

ƷɻؽM_(d)ı͸ײą^(q)ԭHCV-cAgСƂĆο¡w΢װø(bio)ӛHCV-cAgο¡w(yng)Ԍ(du)ѪȽM(yng)pwAø“(lin)߷ԭELISAzy(c)ѪѪ{Ʒб͸ײxĿԭ


ҪMɳ

1

w

96˷1K

2

øY(ji)

20mL1ƿ

3

(yng)Ԍ(du)Ѫ

0.5 mL1ƿ

4

Ԍ(du)Ѫ

0.5 mL1ƿ

5

ϴҺʹÕr(sh)20ϡ?zhun)?/span>

50mL1ƿ

6

 A

6mL2ƿ

7

 B

6mL2ƿ

8

KֹҺ

6mL1ƿ

9

ƷϡҺ

10mL1ƿ

10

3

ע⣺̖ͬ(ho)ĽMֲû


MҪMɣ1w壺ᘌ(du)ĿԭĆο¡w2øY(ji)u׻龏_ҺɽѪţѪ׵ף3(yng)Ԍ(du)Ѫ͸ײĿԭzy(c)(yng)Ѫ4Ԍ(du)Ѫ͸ײĿԭzy(c)ԵѪ5 ϴҺNaClͨ X-1006Aˮc^(gu)7BᣬҶļ׻“(lin)8KֹҺ9ƷϡҺ Tris _ҺNaClҵcţѪ׵œ-20X-100ɽѪ壬  


Ʒ̓x

1rƂsˮȥxˮ

2һ

3_΢Һ͝^

4Ƀˮ

537ˮԡ䣻

6Ӌ(j)r(sh)

7ϴC(j)ϴƿ

8ʎ

9ø(bio)x


(ch)lЧ

28ܹⱣЧ12(g)

a(chn)ڼЧҊ(jin)(bio)


mÃx

Ԅ(dng)ø(bio)y(c)xpL(zhng)r(sh)O(sh)L(zhng)450nmL(zhng)630nmβL(zhng)r(sh)O(sh)Ùzy(c)L(zhng)450nmO(sh)ÿհ׌(du){(dio)


ӱҪ

    1ӱʹѪѪ{cػEDTAȿ(hu)Ӱ푌(sh)(yn)Y(ji)

2ӱo(w)Ƃ̎_t(y)üg(sh)ɼȫѪӱoðСr(sh)Ϻ3000D(zhun)/x10ַxѪʹѪ岻Oٺt׼(x)tܕ(hu)(do)¼(yng)ԽY(ji)

3ʹüӟ̎Ęӱ֬Ѫӱ(yng)(dng)ڌ(sh)(yn)ǰóxķӱ

4S(yn)ȾĘӱ(do)e(cu)`ĽY(ji)

5ӱкЯBc(hu)Ӱ푌(sh)(yn)Y(ji)ïBcӱ

6ѪӱδǰxČ(do)wS׵ĴwS׌(du)Y(ji)Ӱ횴_x̎ǰӱѽ(jng)(du)ڽܿίIJ˘ӱҪL(zhng)r(sh)g


z(yn) 

1(sh)(yn)(zhn)

1حh(hun)ȡԇҜƽ30

2ϴҺƣԇеϴҺsϴҺںmĝ(ni)rƂsˮȥxˮsϴҺϡ20

sϴҺߝ}_Һڜضȵ͕r(sh)ܕ(hu)нY(ji)Ո(qng)?jin)ʹǰ_sϴҺo(w)Y(ji)l(f)F(xin)нY(ji)Ո(qng)ˮԡмӟܽt(hu)ӰϴЧ

2

1ÿΌ(sh)(yn)ڰO(sh)һ(g)հ׿ףO(sh)(yng)Ԍ(du)ոpס

2(yng)Ԍ(du)ѪO(sh)ɿמ(yng)Ԍ(du)ÿ׼(yng)Ԍ(du)Ѫ200L

3Ԍ(du)ѪO(sh)ɿמԌ(du)ÿ׼Ԍ(du)Ѫ200L

4ӘƷϡҺÿף(yng)Ԍ(du)տףм100LƷϡҺհ׿׼200LƷϡҺ

5ӘӣƷÿ׼ƷѪѪ{100L

6òzƬ371ԡʎ60

7ϴ죺СĽҵĤȥ(yng)l׃(ni)ҺwϴҺҺעM(mn)ÿףÿב(yng)벻400LϴҺҺo510ȥ׃(ni)ϴҺ؏(f)5ڸɃˮĸɡ

8øY(ji)ÿ׼200LøY(ji)

9òzƬ371ԡ30

10ϴ죺ͬ7

11@ɫÿȼ100LAټ100LBֻ371ԡܹ@ɫ15

12Kֹ(yng)ÿ׼50LKֹҺֻ

13 Y(ji)y(c)Y(ji)ڷ(yng)Kֹ10犃(ni)M(jn)Мy(c)ʹø(bio)xy(c)ֵxβL(zhng)450nmO(sh)հ׿{(dio)㣩xpL(zhng)L(zhng)450nmL(zhng)630nmxȡODֵ


(yng)Дֵ

CUT OFF_Ԍ(du)ƽֵ+0.06Ԍ(du)ODֵ0.06r(sh)0.06Ӌ(j)㣩

(yng)Ԍ(du)ÿODֵ>0.8Ԍ(du)ODֵÿף0.1r(sh)y(c)Y(ji)Ч

Y(ji)ж

1y(c)ƷODֵCUT OFFжHCVĿԭ(yng)

2y(c)ƷODֵRֵCUT OFFжHCVĿԭ

(du)z(yng)ԵĘƷ(yng)M(jn)p׏(f)zԴ_J(rn)挍(sh)Y(ji)


z(yn)Y(ji)Ľ

1ѪӱδȫѪ{ӱwS׻ӱ(yn)Ⱦ(hu)(du)Y(ji)Ӱ푡

2(sh)(yn)ʹL(zhng)r(sh)gA򷴏(f)ڵĘӱ(hu)(du)ԇęzy(c)`Ӱ푣hʹrӱM(jn)Ќ(sh)(yn)


z(yn)ľ

1ԇЃHڶԙzy(c)ѪѪ{ƷеHCVxĿԭ

2ELISA(yng)ԭԇzy(c)Y(ji)ԲȫųHCVȾĿܣzy(c)Y(ji)(yng)Ա횽Y(ji)ϴ_Cԇָ(bio)RϢM(jn)з


a(chn)Ʒָ(bio)

15I(y)HCVĿԭ(yng)ԅѪzy(c)Y(ji)óF(xin)ԣ4݇(gu)HCVĿԭ(yng)ԅѪzy(c)(yng)ԅƷʣ+/+(yng)3/4

10I(y)(gu)HCVĿԭԅѪzy(c)Y(ji)óF(xin)(yng)4I(y)͙zޅƷzy(c)S1S2 S3(yng)(yng)S45݇(gu)͙zޅƷzy(c)L1L2 L3(yng)(yng)L4pL5(yng)Ի   

I(y)(gu)ҾԅƷ؏(f)10Ιzy(c)п׌(sh)(yn)Y(ji)(yng)CV%15%n=10

(wn)ԇ(yn)M376(xing)ָ(bio)(yng)Ҏ(gu)

2ԇzy(c)͸ײĿԭ`Ȟ1pg/mL

3淴(yng)(sh)(yn)HBVHIVHAV(yng)ԘӱӰ푱ԇЌ(du)HCVĿԭęzy(c)(du)25RFߝ(yng)Ԙӱ23ANAߝ(yng)ԘӱքeñԇM(jn)HCVĿԭzy(c)(li)ӱ(yng)Ԕ(sh)1خԷքe96.0%95.7%

460ͬԴѪͬһߵѪѪ{zy(c)Y(ji)ȫһ


ע(xing)

128lȡĘӱ(yng)Ҝƽ30M(jn)Йzy(c)

2횴_ӘĜ(zhn)_ӘӲ(zhn)_ܕ(hu)(do)e(cu)`Č(sh)(yn)Y(ji)

3ʹ΢ҺֹӘӕr(sh)ÿΑ(yng)ԓQ^ȡӱ

4ڲ^(gu)Б(yng)M΢Юa(chn)

5sϴҺϵߝ}ܕ(hu)γɽY(ji)δȫܽ(hu)Ӱ푌(sh)(yn)Y(ji)ͬr(sh)ܕ(hu)סԄ(dng)ӘϴC(j)ܵF(xin)Y(ji)Ո(qng)37ܽȻϡጾʹ

6z؏(f)ʹ

7ˮԡ偷(yng)r(sh)Ո(qng)(yng)ˮ1/3ײԾW(wng)֧֧Σˮؿ37

8ϴ壺1ϴC(j)ϴr(sh)ϴC(j)ļҺP(gun)ҪϴҺ^(gu)ܳM(mn)(yng)΢ϴΔ(sh)(yng)5(jng)עzҺ^Ƿ

2ֹϴr(sh)Ո(qng)ʹÎмˮİԷԴ^(gu)ø(li)߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zy(c)Y(ji)Ĝ(zhn)_

3ϴr(sh)õˮՈ(qng)𷴏(f)ʹ

4ϴC(j)ÿʹǰsˮȥxˮ_ϴɃԷֹ·g

9κԇ|Ƥw۾ôˮ(du)ԓλM(jn)ДU(ku)ϴKֹҺиg

10ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾՈ(qng)P(gun)Č(sh)(yn)ҹҎ(gu)(li)(zh)к̎


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